CRISPR editing of human cells- hands-on class

This community project will use CRISPR to edit the DNA of human cell lines. We will meet every week on Thursday at 7 PM, but we will only take new members during posted meetup classes (typically the first Thursday of the month). During the first meeting, we will identify which gene we want to knock out and identify the appropriate cell line to make the knockout. In subsequent weeks, we will make CRISPR guide RNAs, clone them into a mammalian expression vector, and then transfect CAS9 with the guide RNA into human cells. The cell lines we generate will be open source and available for any community lab or project.

You do not need any prior experience or knowledge to join. This community project will primarily take place in the laboratory. You must wear close-toed shoes to go into the lab.